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. Author manuscript; available in PMC: 2024 Apr 20.
Published in final edited form as: Mol Cell. 2023 Mar 29;83(8):1251–1263.e6. doi: 10.1016/j.molcel.2023.03.006

Figure 5. The Cbf1 HLH region imparts efficient nucleosome targeting and displacement in vivo.

Figure 5.

(A) and (B) Nucleosome occupancy within the HO promoter that contains a Cbf1 or Pho4 target site, respectively. The Cbf1 and Pho4 target site positions in the −4 nucleosome are indicated by the gray line, respectively. Nucleosome occupancy was determined in the presence (black) or absence of methionine (purple or green), where methionine depletion induces the expression of either Cbf1ΔN, Pho4ΔN, Cbf1ΔN PHLH, or Pho4ΔN CHLHZ. The arrow indicates which TFs shift the position of the −4 nucleosome. (C) Nucleosome occupancy within the HO promoter with the Pho4 target sequence in the same location as panel (B). β-estradiol titrates the expression level of Pho4ΔN (left) or Pho4ΔN CHLHZ (right), respectively. The grey dotted lines indicate the location of the Pho4 target sequence (−775 bp) and the nucleosome center without TF expression (−716 bp). (D) Relative nucleosome occupancy at the Pho4 binding site (−757 bp from the TSS) upon induction of Pho4 ΔN (white) or Pho4 ΔN CHLHZ (black) with increasing levels of β-estradiol. (E) Relative nucleosome occupancy measured at the center of the −4 nucleosome (−716 bp from the TSS) with increasing levels of β-estradiol.