Fig. 3. Proteases-encoding transcripts within the D. gallinae transcriptomes.

a Identification of digestive protease homologues in Dermanyssus gallinae (Dg) transcriptome (highlighted by a red dashed rectangle) and representatives of Chelicerates; Lp, Limulus polyphemus; Pt, Parasteatoda tepidariorum (a representative of Araneae); Tu, Tetranychus urticae; Ss, Sarcoptes scabiei; Is, Ixodes scapularis; Rm, Rhipicephalus microplus; Mo, Metaseiulus occidentalis; Vd, Varroa destructor; Nc, Neoseiulus cucumeris. Green rectangles indicate the presence, and white rectangles indicate the absence of homologue identification with threshold E-values set to ≥1e⁻³⁰. Details of Blast search set-ups and outcomes are shown in Supplementary Fig. S2. b A list of selected proteases and expression values (FPKM) of their mRNA transcripts across libraries derived from individual developmental stages of D. gallinae. Individual accession IDs are available in Supplementary Table S5. UP unfed protonymphs, FP fed protonymphs, FD fed deutonymphs, WB whole bodies. The expression variance for each transcript is indicated by colours ranging from low (blue) to high (red).