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. 2023 May 13;6:517. doi: 10.1038/s42003-023-04907-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Maximum likelihood phylogenetic tree of the arthropod and vertebrate cysLGIC subunit protein sequences. The bootstrap supports above 50% are shown at the main nodes. Dermanyssus gallinae: Dg and IrSig (indicated in red), Tetranychus urticae: Tu, Drosophila melanogaster (D or other), Apis mellifera (Amel), Tribolium castaneum (Tcas), Homo sapiens (Hs), Danio rerio (Dr), Bos taurus (Bt), Equus caballus (Ec), Sus scrofa (Ss), and Gallus gallus (Gg). For accession numbers, see Supplementary Data S1. b FPKM values of individual Acari-specific (Ac) and pH-dependent (pH) chloride channels (Cls) and their midgut/whole body (WB) ratio of adult mites. UP unfed protonymphs, FP fed protonymphs, FD fed deutonymphs. c FPKM values of individual GluCls and their midgut/whole body ratio of adult mites. c’ Dose-dependent viability assays after microinjection of GluCls agonists dissolved in DMSO and diluted to 1% of the solvent. Each data point represents a mean, and SEM of n = 24 or 25 mites per tested concentration. For the source data behind the graphs, see Supplementary Data S1.