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. 2023 May 13;6:516. doi: 10.1038/s42003-023-04905-z

Fig. 4. IL-8 mediates IL-33-induced angiogenic events in HRMVECs.

Fig. 4

a HRMVECs were transfected with control siRNA (siControl) or IL-8 siRNA (siIL-8), after 48 h cell extracts were prepared and analysed for VEGF, and IL-8 levels by Western blotting and normalized to β-tubulin. b–e All the condition were same as in panel a, except that the cells were analyzed for IL-33 (20 ng/mL)-induced migration (b), wound healing (c), tube formation (d), and sprouting assay (e). n = 6 (b, c, d, e) biologically independent samples per group, expressed as Mean ± SD. *P < 0.05 vs siControl, **p < 0.05 vs siControl + IL-33. Scale bar represents 50 μm in panel e.