Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 May 13;14(5):325. doi: 10.1038/s41419-023-05846-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — A, B Western blot analysis of PDHA^s293 (PDH phosphorylated at Ser293 of the E1-α subunit) and total PDHA protein levels in hippocampal lysates from rats of the indicated genotype. Three independent experiments of (A) with at least four male rats of 3-months old per genotype were used for quantification. The PDHA^s293/PDHA level of individual rats is indicated by a dot in (B). The mean ± SEM for each genotype is presented, including WT (n = 6), APP KO (n = 5), SNCA KO (n = 4) and DKO (n = 6). One-way ANOVA was used for statistical analysis, and differences are indicated by asterisks. *p < 0.05 and ***p < 0.001 indicate significant differences, and n.s. indicates no significance. C ATP levels in mitochondria isolated from the rat hippocampi of the indicated genotype. At least four male rats of 3-month-old per genotype were studied in three independent experiments. The data of individual rats are indicated by dots, and the mean ± SEM for each genotype is presented. One-way ANOVA was used for statistical analysis, and differences are indicated by asterisks. *p < 0.05 and ***p < 0.001 indicate significant differences, and n.s. indicates no significance. D Serum lactate levels of rats with the indicated genotype. At least four male rats of 3-month-old per genotype were assayed. The data of individual rats are indicated by dots, and the mean ± SEM for each genotype is presented. One-way ANOVA was used for statistical analysis, and differences are indicated by asterisks **p < 0.01 and ***p < 0.001 indicate significant differences, and n.s. indicates no significance. E, F The protein levels of FL-APP and α-synuclein in the hippocampi of 3-month-old male knockout rats were measured in SNCA KO and APP KO rats by western blotting in (E). Tissue samples were collected from three rats of each genotype. The asterisk indicates a stray band. Quantification is shown in (F) with the mean ± SEM, and each dot represents the data from an individual rat. T-tests were used. *p < 0.05 and ***p < 0.001 indicate significant differences.