Table 1.

Annealing temperatures, time required for primer extension and sequences of oligonucleotide primers used in PCR amplification of bla_NDM-1, PMQRs and integrons

Gene	Sequence (5′-3′)	Size (bp)	Annealing temperature	Time for primer extension	Reference
bla NDM1	F: GGTTTGGCGATCTGGTTTTC R: CGGAATGGCTCATCACGATC	621 bp	55°C	30 Sec.	(23)
qnrB	F: GATCGTGAAAGCCAGAAAGG R: ACGATGCCTGGTAGTTGTCC	469 bp	54°C	30 Sec.	(24)
qnrS	F: CAATCATACATATCGGCACC R: TCAGGATAAACAACAATACCC	641 bp	56°C	30 Sec.	(25)
aac(6′)-Ib	F: TTGCGATGCTCTATGAGTGGCTA R: CTCGAATGCCTGGCGTGTTT	482 bp	58°C	30 Sec.	(26)
Int1	F: CTG CGT TCG GTC AAG GTT CT R: GGA ATG GCC GAG CAG ATC CT	882 bp	58°C	50 Sec.	(27)
Int2	F: CACGGATATGCGACAAAAAGGT R: GTAGCAAACGAGTGACGAAATG	788 bp	54°C	40 Sec.	(28)
Int3	F: GCCTCCGGCAGCGACTTTCAG R: ACGGATCTGCCAAACCTGACT	979 bp	56°C	60 Sec.	(28)

Bp = base pair