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. Author manuscript; available in PMC: 2023 Dec 1.
Published in final edited form as: J Bone Miner Res. 2022 Oct 17;37(12):2498–2511. doi: 10.1002/jbmr.4717

Fig. 4.

Fig. 4.

EGFR signaling promotes the survival, proliferation, and lubrication of chondrocytes in articular cartilage. (A) TUNEL staining of WT and HBEGF OverCol2 articular cartilage at 4 days after 6 N loading. White dashed lines in the images outline uncalcified zone. The percentage of TUNEL+ cells in uncalcified cartilage was quantified and is shown at the right (n = 5/group). Scale bars, 100 μm. (B–E) Ki67 (B), PRG4 (C), MMP13 (D), and ADAMTS5 (E) staining of WT and HBEGF OverCol2 articular cartilage at 2 weeks after 6 N loading. In each panel, the percentage of positive cells in the articular cartilage is quantified at the right (n = 5/group). Scale bars, 50 μm. The comparisons were conducted by two-way ANOVA with Tukey–Kramer multiple comparison test.