Figure 1.

Clustering and identification of atherosclerosis-associated cell states

(A) Schematic overview of the experimental setup.

(B and C) (B) UMAP projection of the scRNA-seq profiles represented as eleven manually annotated clusters. (C) UMAP regional occupancy analysis demonstrating relative changes in cell density comparing atherosclerotic vascular wall to healthy controls. Atherosclerosis-associated cell states are revealed by increased local abundance of cells in regions of the UMAP plot (log2FC, log2 fold change).

(D) UMAP plot depicting the 12 disease-associated cell states and the selected top marker genes.

(E) Relative changes in the cell state proportions during different stages of atherosclerosis shown for each of the three biological replicates. Diamond represents the average of the three replicates.