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. 2002 Apr 1;30(7):1620–1629. doi: 10.1093/nar/30.7.1620

Figure 3.

Figure 3

Figure 3

Interaction of SmpB with tmRNA variants. (A) tmRNA fractionated by centrifugation on a glycerol density gradient was detected by northern hybridization. His-tagged SmpB alone (B) or mixed with wild-type tmRNA (C), 3A (D), 19C (E), 62C (F) 86C (G), 334U (H), pK1L (I), pK2L (J), pK3L (K) or pK4L (L) was fractionated by glycerol density gradient centrifugation and was detected by western blotting using an antibody raised against SmpB. (M) Mutations designated on a secondary structure model of E.coli tmRNA. The tag-encoded sequence highlighted by white with a black background is surrounded by four pseudoknots (PK1–PK4). Non-Watson–Crick base pairs are shown by open circles. This RNA has two tRNA-specific modified nucleotides, 5-methyl U and pseudouridine in the T-loop (6), indicated as T and Ψ, respectively. Arrows indicate the mutations used in this study.