Table 2.
Basic characteristics of offspring.
| Female mice |
17 days after birth |
|||||||
|---|---|---|---|---|---|---|---|---|
| AIN |
LP |
Interaction |
||||||
| Number | 7 | 13 | P | |||||
| Body weight (g) | 11.6 | ± | 0.480 | 9.97 | ± | 0.400 | * | |
| Duodenum weight (g) | 0.08 | ± | 0.008 | 0.07 | ± | 0.003 | ||
| Jejunum weight (g) | 0.23 | ± | 0.013 | 0.21 | ± | 0.008 | ||
| Ileum weight (g) | 0.14 | ± | 0.006 | 0.12 | ± | 0.005 | * | |
| Cecum weight (g) | 0.09 | ± | 0.040 | 0.04 | ± | 0.004 | ||
| Colon weight (g) | 0.11 | ± | 0.005 | 0.09 | ± | 0.005 | ||
| Serum corticosterone (ng/mL) | 4.61 | ± | 0.404 | 4.54 | ± | 0.450 | ||
| 21 days after birth |
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AIN |
LP |
Interaction |
||||||||||||
| NW |
EW |
NW |
EW |
P |
||||||||||
| Number | 5 | 6 | 17 | 12 | ||||||||||
| Body weight (g) | 15.1 | ± | 0.52 | 15.4 | ± | 0.51 | 13.9 | ± | 0.50 | 11.4 | ± | 0.92 | ## | |
| Duodenum weight (g) | 0.15 | ± | 0.009 | 0.14 | ± | 0.009 | 0.12 | ± | 0.005 | 0.11 | ± | 0.009 | ||
| Jejunum weight (g) | 0.35 | ± | 0.009 | 0.37 | ± | 0.009 | 0.34 | ± | 0.150 | 0.29 | ± | 0.023 | ||
| Ileum weight (g) | 0.16 | ± | 0.002 | 0.19 | ± | 0.002 | 0.17 | ± | 0.008 | 0.15 | ± | 0.012 | ||
| Cecum weight (g) | 0.06 | ± | 0.005 | 0.11 | ± | 0.005 | 0.06 | ± | 0.004 | 0.05 | ± | 0.005 | ||
| Colon weight (g) | 0.15 | ± | 0.007 | 0.16 | ± | 0.007 | 0.16 | ± | 0.005 | 0.14 | ± | 0.009 | ||
| Corticosterone (ng/mL) |
1.97 |
± |
0.413 |
3.71 |
± |
0.627 |
3.80 |
± |
0.429 |
2.60 |
± |
0.352 |
0.0096 |
|
| 28 days after birth |
||||||||||||||
| AIN |
LP |
Interaction | ||||||||||||
| NW |
EW |
NW |
EW |
P |
||||||||||
| Number |
5 |
7 |
17 |
12 |
||||||||||
| Body weight (g) | 25.8 | ± | 0.92 | 25.1 | ± | 0.72 | 24.3 | ± | 0.69 | 21.3 | ± | 1.76 | # | |
| Duodenum weight (g) | 0.17 | ± | 0.008 | 0.19 | ± | 0.009 | 0.20 | ± | 0.003 | 0.18 | ± | 0.012 | 0.019 | |
| Jejunum weight (g) | 0.53 | ± | 0.027 | 0.54 | ± | 0.028 | 0.51 | ± | 0.024 | 0.47 | ± | 0.039 | ||
| Ileum weight (g) | 0.28 | ± | 0.014 | 0.28 | ± | 0.019 | 0.28 | ± | 0.012 | 0.25 | ± | 0.015 | ||
| Cecum weight (g) | 0.08 | ± | 0.010 | 0.09 | ± | 0.009 | 0.13 | ± | 0.029 | 0.08 | ± | 0.006 | ||
| Colon weight (g) | 0.23 | ± | 0.012 | 0.21 | ± | 0.013 | 0.21 | ± | 0.008 | 0.19 | ± | 0.009 | ||
| Corticosterone (ng/mL) | 1.33 | ± | 0.173 | 2.45 | ± | 0.410 | 2.42 | ± | 0.173 | 1.98 | ± | 0.396 | ||
Abbreviations: AIN, AIN-93G; LP, low-protein, EW, early weaning; NW, normal weaning. Values are expressed as means ± SEM for 5–17 mice. Seventeen days after birth: *P < 0.05 vs. AIN-93G, according to the Student's t-test. Twenty-one and twenty-eight days after birth: #P < 0.05 vs. LP-NW, ##P < 0.01 vs. LP-NW according to the two-way analysis of variance (Tukey's test).