Figure 1.
SDS–PAGE of cell lysates and purified proteins. (A) Aliquots of extracts from High Five cells infected with baculovirus UL56 (cell extract) and rpUL56 obtained after a two-step purification using anion exchange followed by gel permeation chromatography (purif. rpUL56) were subjected to SDS–PAGE prior to staining with silver. (B) Aliquots after purification (purif. rpUL56) and following spin concentration (conc. rpUl56) were subjected to SDS–PAGE prior to staining with Coomassie brilliant blue. (C) Aliquots of extracts from High Five cells infected with baculovirus UL89 (cell extract) and rpUL89 obtained after two-step purification using anion exchange followed by gel permeation chromatography (purif. rpUL89) were subjected to SDS–PAGE prior to staining with silver. (D) Aliquots after purification (purif. rpUL89) and following spin concentration (conc. rpUL89) were subjected to SDS–PAGE prior to staining with Coomassie brilliant blue. Molecular weight markers (M) are indicated on the left; the positions of the proteins are indicated on the right.