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. 2002 Apr 1;30(7):1695–1703. doi: 10.1093/nar/30.7.1695

Figure 4.

Figure 4

Nuclease activity of HCMV rpUL56 and rpUL89. (A) Nuclease assay with purified proteins. Lane 1, pUC-aseq; lane 2, incubation in the presence of HindIII; lane 3, pUC-aseq incubated with purified rpUL56; lane 4, pUC-aseq incubated with purified rpUL89. (B) Nuclease assay with pBR322 as substrate. Lane 1, plasmid pBR322 in the absence of protein; lane 2, pBR322 with the restriction endonuclease HindIII; lane 3, pBR322 incubated with purified rpUL56; lane 4, pBR322 incubated with purified rpUL89; lane 5, incubation with purified MSP1ΔA; lane 6, pBR322 incubated with wild-type baculovirus-infected extracts; lane 7, pBR322 incubated in the presence of mock-infected extracts. The arrows indicate three different plasmid DNA forms: open circular molecules (oc), linear forms (lin) and supercoiled DNA (sc).