Table 1.
List of antibodies used for IHC.
| Primary antibodies | Host species | Dilution | Supplier and Cat. No |
|---|---|---|---|
| Anti-NKCC1, GpA (aa 237–275 of mouse NKCC1), affinity purified polyclonal | guinea pig | 1:300 or 1:1000 | Dr Masahiko Watanabe, custom-made |
| Anti-NKCC1, RbC (aa 1108–1141 of mouse NKCC1), affinity purified polyclonal | rabbit | 1:300 or 1:1000 | Dr Masahiko Watanabe, custom-made (Wei et al. 2013) |
| Anti-NKCC1a (aa 977–991 of mouse NKCC1), serum | rabbit | 1:300 or 1:1000 | Dr Christian Hübner, custom-made (Göppner et al. 2019) |
| Anti-NKCC1a (aa 977–991 of mouse NKCC1), affinity purified polyclonal Anti-beta-IV-spectrin |
rabbit rabbit |
1:300 or 1:1000 1:500 |
Dr Christian Hübner, custom-made (Göppner et al. 2019) Dr Matthew N. Rasband, custom-made (Yang et al. 2004) |
| Anti-CD13, polyclonal | goat | 1:500 | AF2335, R&D Systems |
| Anti-CNPase, monoclonal | mouse | 1:2000 or 1:5000 | AMAb91072, Atlas Antibodies |
| Anti-DCX, polyclonal | chicken | 1:200 or 1:500 | Ab153668, Abcam |
| Anti-GFAP, affinity purified polyclonal | chicken | 1:500 | Ab5541, EMD Millipore Corp. |
| Anti-Iba1, polyclonal | rabbit | 1:500 | 019–19,741, Fujifilm Wako |
| Anti-MAP2, polyclonal | rabbit | 1:250 | Ab5622, EMD Millipore Corp. |
| Anti-MBP, polyclonal | chicken | 1:5000 | PA1–10008, Invitrogen |
| Anti-Olig2, polyclonal | goat | 1:200 | AF2418, R&D Systems |
| Anti-PDGFRalpha (D1E1E), monoclonal | rabbit | 1:500 | #3174, Cell Signaling Technology |
| Anti-VGAT, polyclonal | rabbit | 1:1000 | 131–002, Synaptic Systems |
| Anti-VGLUT1, polyclonal | guinea pig | 1:3000 | Ab5905, EMD Millipore Corp. |
| Anti-VGLUT2, polyclonal | guinea pig | 1:300 | 135–404, Synaptic Systems |
| Secondary antibodies | Host species | Dilution | Supplier and Cat. No |
| Anti-chicken-Alexa633 | goat | 1:1000 | A21103, Invitrogen |
| Anti-guinea pig-Alexa488 | goat | 1:1000 | Ab150185, Abcam |
| Anti-guinea pig-Alexa568 | goat | 1:1000 | A11075, Invitrogen |
| Anti-mouse-Alexa488 | goat | 1:1000 | A11001, Life Technologies |
| Anti-rabbit-Alexa488 | goat | 1:1000 | A11034, Life Technologies |
| Anti-rabbit-Alexa568 | goat | 1:1000 | A11036, Life Technologies |
| Anti-goat-Alexa488 | donkey | 1:1000 | Ab150129, Abcam |
| Anti-rabbit-Alexa568 | donkey | 1:1000 | A10042, Life Technologies |