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. 2023 Mar 10;46(5):298–308. doi: 10.14348/molcells.2023.2148

Fig. 3. Knockdown of TNS4 reduced colony formation and cell migration in gastric cancer (GC) cells.

Fig. 3

(A) Establishment of TNS4 knockdown cells. GC cell lines (SNU-601, KATO III, and MKN74) were transduced with TNS4-specific shRNA or an empty shRNA vector (shCtrl). TNS4 mRNA and protein levels were measured by qRT-PCR (top) and western blotting (bottom), respectively. β-Actin was used as the loading control. (B) Colony formation assay of control and TNS4 knockdown GC cells. (C) Cell migration assay of control and TNS4 knockdown GC cells at 24 h after seeding. Migration relative to that of the control cells is shown. In (B) and (C), n = 3 independent experiments; mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001 (Mann–Whitney U-test).