Fig. 6. Simoa-based quantification of Ras baseline levels of OVCAR-3 cells. Calibration curves of Ras Simoa assay at different (a) Ras concentrations or (b) OVCAR-3 cell densities. The correlation between Ras concentrations and cell densities. Ras concentrations from Ras recombinant proteins or cell lysates were measured by Ras Simoa assay. Data are means ± SD of two independent experiments.