Scheme 1. Scheme of the nanoSimoa pipeline, which integrates cancer nanomedicine to treat cancer cells and single molecule-array (Simoa) to profile protein level changes in response to the nanomedicine. Specifically, PEGylated mesoporous silica nanoparticles (MSN-PEG) carrying topotecan (TOP), a water-soluble drug used to treat various cancers, are utilized in this study. The nanoparticles are ultrasound-responsive, and their release of TOP molecules is triggered by ultrasound stimulation inside cancer cells. To evaluate the cellular responses to the nanomedicine, cell viability is measured, and Simoa assays are used to profile protein biomarkers. If the results are unsatisfactory, we will redesign the nanomedicine and repeat the nanoSimoa pipeline. Moreover, nanoSimoa can assess multiple nanomedicines in parallel.