Figure EV2. C53 orthologous from two TSAR representatives as well as representatives from Haptista, Cryptista, Diplomonada, Amoebozoa, and CRuMs interact with human and Arabidopsis ATG8 and UFM1.

Bacterial lysates containing recombinant protein were mixed and pulled down with glutathione magnetic agarose beads. Input and bound proteins were visualized by immunoblotting with anti‐GST and anti‐MBP antibodies. Tetrahymena thermophila (Tt), Dictyostelium purpureum (Dp), Emiliania huxleyi (Eh), Guillardia theta (Gt), Trypanosoma brucei (Tb), Rigifila ramosa (Rr) and Phytophthora sojae (Ps).