Figure 3. Auxin inducible S6K1 phosphorylation via TOR depends on the activation of glycolysis in the primary root.

• A
  Representative western blot of root tissues of pUB10:S6K1‐3xHA treated by the indicated combination of auxin (IAA, 10 μM), sucrose (Suc, 110 mM), 2‐deoxy‐d‐glucose (2D, 20 mM) and AZD8055 (AZD, 10 μM) and probed with anti‐S6K1/2, anti‐HA or anti‐S6K1‐T449P. Blot is one of three biological replicates.
• B
  Quantification of the relative S6K activation. Box plots show three biological replicates, and comparison between samples was performed by one‐way ANOVA and post‐hoc Tukey HSD Test (α = 0.05); different letters indicate significant differences.
• C
  Representative DIC images showing RPT1B promoter expression at different stages of LR development in 10 DAG seedlings. Orange arrowheads indicate the accumulation of reporter signals in the dividing lateral root founder cells. Scale bars: 50 μm.
• D
  Representative confocal section showing S6K1 expression in different stages of LR development in 10 DAG pS6K1:gS6K1‐CFP seedlings. Orange arrowheads indicate the accumulation of reporter signals in the dividing lateral root founder cells. Scale bars: 50 μm.

Source data are available online for this figure.