Figure 4. TORC is required in the pericycle for lateral root formation.

• A–C
  Box plots of emerged LR in TOR‐oe (A), rpt1b (B), and lst8 (C) at 14 DAG. Comparison between samples was performed by one‐way ANOVA and post‐hoc Tukey HSD Test (α = 0.05); different letters indicate significant differences. The number of roots scored is indicated.
• D
  Schematic of the experimental setup used for scoring LR formation by gravistimulation upon inhibition of TOR by AZD8055 (10 μM).
• E
  Proportion of bends developing lateral root primordia after transfer to AZD8055 containing media and gravistimulation for 24 h (n = 10).
• F
  Representative confocal images of calcofluor counterstained bends of 7DAG UB10pro>>amiR‐TOR seedlings following a 24 h pre‐treatment with mock (DMSO) or ß‐Estradiol (10 μM) and subsequent 24 h gravistimulation (ep: epidermis, co: cortex, en: endodermis, pe: pericycle, px: protoxylem). Numbers indicate the proportion of root bends with the depicted phenotype. Scale bar: 50 μm.
• G
  Representative DIC images of bends in 7 DAG UB10pro>>amiR‐TOR/DR5:GUS seedlings stained for GUS activity after a 24 h pre‐treatment with mock (DMSO) or ß‐Estradiol (Est, 10 μM) and subsequent 24 h gravistimulation. The proportion of root bends showing the depicted phenotype is indicated. Scale bar: 100 μm.
• H
  Fraction of bends developing lateral root primordia and stained for GUS activity in primary root vasculature of UB10pro>>amiR‐TOR/DR5:GUS, n = 18 bends.

Source data are available online for this figure.