Skip to main content
. 2023 Apr 6;42(10):e111273. doi: 10.15252/embj.2022111273

Figure 6. TOR is required for aARF7/ARF19 independent formation of adventitious roots via WOX11.

Figure 6

  • A
    Abundance of WOX11 transcripts in RNAseq samples. mRNA accumulation in response to auxin is elevated when TOR is knocked down. n = 3 biological replicates.
  • B
    Distribution of GUS‐staining in UB10pro>>amiR‐TOR/WOX11pro::GUS seedlings 6 days after transfer to 10 μM Est. The proportion of root bends with the depicted phenotype is indicated.
  • C
    The arf7/arf19 mutant shows defective LR initiation.
  • D
    Formation of roots (arrows) close to the wound site of arf7/arf19 primary roots 6 days after excision (DAE).
  • E
    Blocking TOR‐activity via exposure to 10 μM AZD8055 (AZD) resulted in the loss of rooting capability on the wound site of arf7/arf19 primary roots at 6 DAE. Scale bars = 3 mm.
  • F, G
    Close‐ups indicate root formation location or absence of root formation on ctrl media and AZD‐containing media, respectively, in arf7/arf19 primary roots at 6 DAE. The proportion of root wound sites with the depicted phenotype is indicated. Scale bars = 100 μm.
  • H
    Model for TOR dependency of root branching in Arabidopsis.

Source data are available online for this figure.