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. 2002 Apr 1;30(7):1593–1605. doi: 10.1093/nar/30.7.1593

Figure 3.

Figure 3

PriABs is required for ssiA activity and pAMβ1-type plasmid replication. (A) Bacillus subtilis PPBJ65 (PriABs+) and PPBJ69 (priAind) strains harbouring pC194-derived plasmid pADG6406-1 (ssiA+) or pADG6406-2 (ssiA–) were grown overnight in LB supplemented with IPTG, Em and Cm, then diluted 100-fold in fresh medium without IPTG and cultivated for ∼5 h. Total DNA was extracted and analysed by Southern hybridisation using 32P-labelled pC194 DNA as probe. (B) Bacillus subtilis PPBJ65 (PriABs+) and PPBJ69 (priAind) strains harbouring pAMβ1-derived plasmid pVA798ΔRCR were grown to mid-log phase without IPTG and their total DNA was extracted and analysed by Southern hybridisation, using 32P-labelled pVA798ΔRCR as probe. ssDNA and dsDNA, single-stranded and double-stranded DNA, respectively.