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. Author manuscript; available in PMC: 2023 May 15.
Published in final edited form as: Circ Res. 1999 May 14;84(9):1020–1031. doi: 10.1161/01.res.84.9.1020

Figure 9.

Figure 9.

PKA inhibition with Rp-8-CPT-cAMPS (100 μmol/L) completely abolishes the positive contractile responses of SNAP and NOC5. A, Representative example of the effect of SNAP (1 μmol/L) in the continued presence of inhibitors of GC (ODQ 10 μmol/L) and PKA (Rp-8-CPT-cAMPS 100 μmol/L) on myocyte contraction and [Ca2+]i. The continuous chart recording shows the failure of SNAP (1 μmol/L) to increase the isotonic twitch contraction in a cardiac myocyte pretreated and continuously perfused with ODQ and Rp-8-CPT-cAMPS. The bottom tracings show, on an expanded time scale, [Ca2+]i and cell length at the times indicated (a, baseline; b, 10 minutes after addition of SNAP; and c, 15 to 20 minutes after SNAP). Under these conditions, SNAP had no significant effect on either contraction amplitude or Cai amplitude. Representative examples of the lack of effect on myocyte contraction of SNAP (1 μmol/L) (B), NOC5 (1 μmol/L) (C), and NOC5 (1 μmol/L) pretreated with the GC inhibitor ODQ (10 μmol/L) (D), each in the continued presence of the inhibitor of PKA (Rp-8-CPT-cAMPS 100 μmol/L).