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. 2023 Apr 29;63:102711. doi: 10.1016/j.redox.2023.102711

Fig. 3.

Fig. 3

NFATc1 promoted the transcription of SLC7A11 during osteoclastogenesis

(a and b), western blot analysis of NFATc1, Nrf2, Keap1, SLC7A11, and HO-1 protein levels during osteoclast differentiation. n = 3 per group. c, RT-qPCR analysis of Nfatc1 in BMDMs, pre-OCs, and ma-OCs. n = 3 per group. (d and e), western blot analysis of NFATc1 and SLC7A11 protein levels in empty vector (EV) and NFATc1 overexpression pre-OCs. n = 3 per group. f, RT-qPCR analysis of Nfatc1 and Slc7a11 in empty vector (EV) and NFATc1 overexpression pre-OCs. n = 3 per group. (g and h), western blot analysis of NFATc1 and SLC7A11 protein levels in siControl (siCTL) and siNFATc1 pre-OCs. n = 3 per group. i, RT-qPCR analysis of Nfatc1 and Slc7a11 in siControl (siCTL) and siNFATc1 pre-OCs. n = 3 per group. j, heatmap for the distribution of NFATc1 binding sites detected by CUT&Tag assay in pre-OCs. TSS transcription, start site, TES, transcription end site. k, Gene Ontology (GO) analysis of the genes near the peaks. l, genomic binding pattern of the Slc7a11 locus showing the NFATc1-binding locations. m, RT-qPCR analysis using Primers 1 and Primers 2 of Slc7a11 in BMDMs and pre-OCs. n = 3 per group. n, RT-qPCR analysis using Primers 1 and Primers 2 of Slc7a11 in EV and NFATc1 overexpression in BMDMs. n = 3 per group. o, Dual-Luciferase reporter assay conducted in MC3T3-E1 cell line. n = 3 per group. All data in this figure are represented as mean ± SD. ns, no significance, **P < 0.01, ***P < 0.001, ****P < 0.0001.