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. 2023 Apr 11;14(5):621–628. doi: 10.1021/acsmedchemlett.3c00047

Table 1. Comparison of Assay Enzymes and Preliminary Screening Data.

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a

Data are the means of measurements in three technical replicates.

b

[Compound] = 250 μM, [ThDP] = 50 μM.

c

[Compound] = 1.5 mM, [ThDP] = 0.3 mM; compounds insufficiently soluble were excluded. (The KM of yeast PDC for ThDP was found to be 11–23 μM, so a high [ThDP] was needed to fully saturate the enzyme.)