Skip to main content
. 2023 May 15;14:2782. doi: 10.1038/s41467-023-38108-1

Table 1.

cryo-EM data collection, map, and model refinement statistics

(DH270.UCA) (DH270.UCA.G57R-CH848.10.17DT) (DH270.I5.6-CH848.10.17) (DH270.I4.6-CH848.10.17) (DH270.I3-CH848.10.17) (DH270.I2-CH848.10.17) (DH270.I1.6-CH848.10.17) (DH270.1-CH848.10.17) (DH270.2-CH848.10.17) (DH270.3-CH848.10.17)
Data Collection
Microscope FEI Titan Krios
Detector Gatan K3
Magnification 105000
Voltage (kV) 300
Electron dose (e-2) 71 69 53 60 60 60 60 60 60 60
Pixel Size (Å) 1.06 1.066 1.066 1.08 1.066 1.066 1.04 1.066 1.066 1.066
Defocus Range (µm) ~0.8-4.3 ~0.7-4.1 ~0.5-4.1 ~0.5-3.1 ~0.5-3.5 ~0.5-3.5 ~0.5-4 ~1.5-3.5 ~0.5-4 ~0.5-3.5
Reconstruction software cryoSPARC
Symmetry C3 C3 C3 C3 C3 C3 C3 C3 C3 C3
Particles 120981 208426 101922 338,934 67110 39612 65522 125676 24465 76274
Box size (pix) 384 384 384 300 384 384 384 384 384 384
Resolution (Å) (FSC0.143)a 4.0 3.3 3.9 4.2 4.3 3.5 3.9 3.6 4.1 3.4
Coordinate Refinement (Phenix)b
Protein residues 2496 2490 2490 2490 2490 2490 2490 2490 2490 2490
R.M.S. deviations
Bond lengths (Å) 0.006 0.006 0.006 0.007 0.006 0.006 0.006 0.013 0.006 0.006
Bond angles (°) 0.908 0.892 0.909 0.987 0.898 0.990 0.885 1.159 0.926 0.933
Validation
Molprobity score 1.42 1.48 1.39 1.48 1.57 1.56 1.42 1.44 1.54 1.52
Clash score 2.15 2.86 2.30 2.56 3.06 3.95 2.59 2.91 2.54 2.98
Favored rotamers (%) 99.77 99.53 99.72 99.63 99.67 99.21 99.86 99.72 99.53 99.68
EMRinger Score 2.12 2.06 2.28 1.81 2.73 2.53 2.61 2.23 1.39 1.63
Ramachandran plot
Favored regions (%) 93.41 94.04 94.4 93.11 92.34 94.48 94.44 94.77 91.65 93.31
Disallowed regions (%) 0.2 0.36 0.12 0.24 0.24 0.16 0.36 0.32 0.49 0.28

a Resolutions are reported according to the FSC 0.143 gold-standard criterion.

b Statistics are reported for the protein residues within the complex excluding the antibody constant domains.