Table 1.
(DH270.UCA) | (DH270.UCA.G57R-CH848.10.17DT) | (DH270.I5.6-CH848.10.17) | (DH270.I4.6-CH848.10.17) | (DH270.I3-CH848.10.17) | (DH270.I2-CH848.10.17) | (DH270.I1.6-CH848.10.17) | (DH270.1-CH848.10.17) | (DH270.2-CH848.10.17) | (DH270.3-CH848.10.17) | |
---|---|---|---|---|---|---|---|---|---|---|
Data Collection | ||||||||||
Microscope | FEI Titan Krios | |||||||||
Detector | Gatan K3 | |||||||||
Magnification | 105000 | |||||||||
Voltage (kV) | 300 | |||||||||
Electron dose (e-/Å2) | 71 | 69 | 53 | 60 | 60 | 60 | 60 | 60 | 60 | 60 |
Pixel Size (Å) | 1.06 | 1.066 | 1.066 | 1.08 | 1.066 | 1.066 | 1.04 | 1.066 | 1.066 | 1.066 |
Defocus Range (µm) | ~0.8-4.3 | ~0.7-4.1 | ~0.5-4.1 | ~0.5-3.1 | ~0.5-3.5 | ~0.5-3.5 | ~0.5-4 | ~1.5-3.5 | ~0.5-4 | ~0.5-3.5 |
Reconstruction software cryoSPARC | ||||||||||
Symmetry | C3 | C3 | C3 | C3 | C3 | C3 | C3 | C3 | C3 | C3 |
Particles | 120981 | 208426 | 101922 | 338,934 | 67110 | 39612 | 65522 | 125676 | 24465 | 76274 |
Box size (pix) | 384 | 384 | 384 | 300 | 384 | 384 | 384 | 384 | 384 | 384 |
Resolution (Å) (FSC0.143)a | 4.0 | 3.3 | 3.9 | 4.2 | 4.3 | 3.5 | 3.9 | 3.6 | 4.1 | 3.4 |
Coordinate Refinement (Phenix)b | ||||||||||
Protein residues | 2496 | 2490 | 2490 | 2490 | 2490 | 2490 | 2490 | 2490 | 2490 | 2490 |
R.M.S. deviations | ||||||||||
Bond lengths (Å) | 0.006 | 0.006 | 0.006 | 0.007 | 0.006 | 0.006 | 0.006 | 0.013 | 0.006 | 0.006 |
Bond angles (°) | 0.908 | 0.892 | 0.909 | 0.987 | 0.898 | 0.990 | 0.885 | 1.159 | 0.926 | 0.933 |
Validation | ||||||||||
Molprobity score | 1.42 | 1.48 | 1.39 | 1.48 | 1.57 | 1.56 | 1.42 | 1.44 | 1.54 | 1.52 |
Clash score | 2.15 | 2.86 | 2.30 | 2.56 | 3.06 | 3.95 | 2.59 | 2.91 | 2.54 | 2.98 |
Favored rotamers (%) | 99.77 | 99.53 | 99.72 | 99.63 | 99.67 | 99.21 | 99.86 | 99.72 | 99.53 | 99.68 |
EMRinger Score | 2.12 | 2.06 | 2.28 | 1.81 | 2.73 | 2.53 | 2.61 | 2.23 | 1.39 | 1.63 |
Ramachandran plot | ||||||||||
Favored regions (%) | 93.41 | 94.04 | 94.4 | 93.11 | 92.34 | 94.48 | 94.44 | 94.77 | 91.65 | 93.31 |
Disallowed regions (%) | 0.2 | 0.36 | 0.12 | 0.24 | 0.24 | 0.16 | 0.36 | 0.32 | 0.49 | 0.28 |
a Resolutions are reported according to the FSC 0.143 gold-standard criterion.
b Statistics are reported for the protein residues within the complex excluding the antibody constant domains.