Figure 6.

IC₅₀of WIN18,446 for inhibition of 11-COOH-THC formation in HLS9. 11-OH-THC (3 μM) was incubated with HLS9 (0.1 mg/mL) and NAD⁺ (2 mM) with the ALDH inhibitor WIN (0.03–300 μM) for 10 min. The estimated IC₅₀ of WIN (>60 μM) was calculated by fitting a nonlinear dose–response inhibition model to the data in GraphPad Prism. Points represent the mean ± standard deviation of three replicates from a single experiment, and curves were calculated from two independent experiments (R1 and R2) completed across two different days.