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. 2023 May 10;16(6):dmm050053. doi: 10.1242/dmm.050053

Fig. 3.

Fig. 3.

RSPO1 overexpression induces the expansion of canonical WNT activity zone in the adrenal cortex in a sexually dimorphic manner. (A) Immunofluorescence staining for β-catenin using adrenal sections from 4-week-old mice. Scale bar: 50 μm (top panels); 20 μm (bottom panels). (B,C) In situ hybridization using the RNA Scope technology for Axin2 (B), a target of canonical WNT signaling, and Wnt4 (C) at 4 weeks of age. Insets represent high-power images of the outer cortex in GOF animals. The outer cortex is denoted by a dotted line in B (female control; top left panel). Scale bars: 50 μm. (D,E) Graphs showing the mean number of Axin2 (D) and Wnt4 (E) transcripts per cell in the outer cortex or inner cortex, based on RNA Scope-based detection on adrenal sections from 4-week-old mice (error bars represent s.e.m., whereas each dot represents an independent biological replicate). The numbers above the graph columns represent number of samples in each group (n). Statistical analysis was performed using unpaired two-tailed t-test. P-value comparing male versus female outer cortex for Axin2=0.0058. P-value comparing male versus female inner cortex=0.1302. P-value comparing male versus female outer cortex for Wnt4=0.1963. P-value comparing male versus female inner cortex=0.0301. ns, not significant; *P<0.05; **P<0.01.