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. Author manuscript; available in PMC: 2023 May 15.
Published in final edited form as: Nature. 2022 Nov 30;613(7945):729–734. doi: 10.1038/s41586-022-05587-z

Figure 4. UptA and PopT are broadly conserved UndP transporters.

Figure 4

(a) Representative microscopy images of the indicated B. subtilis or S. aureus strains. Two strains expressing different fluorescent proteins (B. subtilis) or labeled with different fluorescent D-amino acids (S. aureus) were mixed and then stained with fluorescent MX2401 (MX2401-FL). The left panels show overlays of phase-contrast and fluorescent images in the red and blue channels to distinguish the two strains. The right panels show MX2401-FL staining. Yellow carets highlight wild-type cells or cells over-expressing UptA(Bs) or PopT(Sa). White carets highlight cells lacking the UndP transporters. Scale bar, 1μm. MIC assays of B. subtilis strains over-expressing UptA (b) or PopT (c) homologs from the indicated bacteria.