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Dose-dependent and specific inhibition of gene expression by siRNA. (A) Concentration-dependent effect of siRNA. PCS2-Luc (150 pg) was co-injected with the indicated concentrations of GL3 siRNA. The injection volume was 5 nl. (B) Representative examples of uninjected and GL3 siRNA-injected embryos at stages 8.5, 16 and 20. siRNA (20 µM in 5 nl) was injected at the 2-cell stage. At least 40 embryos were compared.
