Figure 4.

Quantitative analysis of the activities of six homing endonuclease variants of I-ScaI and PI-SceI. Cells harboring pBar2-PI-SceI-site (left three bars) or pBar2-I-ScaI-site (right three bars) were transformed with the pSupE-nuclease plasmids encoding the six nucleases listed and processed as described in the Materials and Methods. The percentage of surviving colonies on arabinose-containing media relative to the number of colonies arising from an identical number of transformants plated on glucose-containing media is shown for each nuclease. Values reflect the average of three independent trials and standard deviations were <15% of each value reported.