Video 1. Conformational rearrangement and distinct coordination geometry adopted by DraNramp during Mn²⁺ import.

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The global conformational changes are highlighted first, with cartoon representations of each of the six structures in the Mn²⁺ transport cycle starting from the outward-open Mn²⁺-bound conformation rotating through the occluded Mn²⁺-bound, inward-open Mn²⁺-bound, and then the metal-free states transitioning back to outward-open Mn²⁺-bound form. The second set of scenes then focusses on the orthosteric site, cycling through the structures in the same order to illustrate the distinct coordination geometries of the bound Mn²⁺, and the corresponding positions of its interacting ligands (protein residues and water) in the metal-free states.