Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Antibody to PUMA (polyclonal) | Cell Signaling Technology | Cat. #: 4976 S; RRID: AB_2064551 | Dilution (1:400) |
| Antibody | Alexa-488 anti-Rabbit IgG secondary antibody (polyclonal) | Abcam | Cat: # ab150077; RRID: AB_2630356 | Dilution (1:1000) |
| Cell line (M. musculus) | Baby Mouse Kidney (BMK)- WT | PMID:11836241 | Dr. Eileen White (Rutgers University) | |
| Cell line (M. musculus) | Baby Mouse Kidney (BMK)- DKO | PMID:11836241 | Dr. Eileen White (Rutgers University) | |
| Cell line (M. musculus) | NMuMG | RRID: CVCL_0075 | Dr. Jeff Wrana (University of Toronto) | |
| Cell line (H. sapiens) | HEK293 | Other (Graham et al., 1977) | RRID: CVCL_0045 | Provided by Dr. Frank Graham (McMaster University). |
| Cell line (H. sapiens) | HCT-116 | Other (Polyak et al., 1996) | RRID: CVCL_0291 | Provided by Dr. Bert Vogelstein (John Hopkins University). |
| Cell line (H. sapiens) | MCF-7 | PMID:3790748 | RRID: CVCL_0031 | Provided by Dr. Ronald N. Buick (University of Toronto) |
| Chemical compound, drug | DRAQ5 | ThermoFisher Scientific, Molecular probes | Cat. #62251 | Far red nucleic acid specific fluorescent dye for cell imaging |
| Chemical compound, drug | MitoTracker Red | ThermoFisher Scientific, Molecular probes | Cat. #: M22425 | Mitochodria specific fluorescent dye |
| Chemical compound, drug | BODIPY FL thapsigargin | MedChemExpress | Cat. #: HY-D1608 | ER specific fluorescent dye |
| Chemical compound, drug | Alexa 647-maleimide | ThermoFisher Scientific, Molecular probes | Cat. #: A20347 | Thiol reactive fluorescent dye |
| Chemical compound, drug | Alexa568-maleimide | ThermoFisher Scientific, Molecular probes | Cat. #. A20341 | Thiol reactive fluorescent dye |
| Chemical compound, drug | PC (L-α-phosphatidylcholine) | Avanti Polar Lipids | Cat. #:840051 C | for making liposomes, used 48% PC |
| Chemical compound, drug | DOPS (1,2-dioleoyl-sn-glycero-3-phospho-L-serine) | Avanti Polar Lipids | Cat. #: 840035 C | for making liposomes, used 10% DOPS |
| Chemical compound, drug | PI (L-α-phosphatidylinositol) | Avanti Polar Lipids | Cat. #:840042 C | for making liposomes, used 10% PI |
| Chemical compound, drug | PE (L-α-phosphatidylethanolamine) | Avanti Polar Lipids | Cat. #: 841118 C | for making liposomes, used 28% PE |
| Chemical compound, drug | TOCL, (18:1 Cardiolipin) | Avanti Polar Lipids | Cat. #: 710335 C | for making liposomes, used 4% TOCL |
| Chemical compound, drug | A-1331852 | Chemietek | Cat. #: CT-A115 | in DMSO |
| Chemical compound, drug | S-63845 | Chemietek | Cat. #: 1799633-27-4 | in DMSO |
| Chemical compound, drug | S-64315 also named “MIK665” | ChemieTek | Cat. # CT-MIK665 | in DMSO |
| Chemical compound, drug | Navitoclax; ABT-263 | Selleckchem | Cat. #: S1001 | in DMSO |
| Chemical compound, drug | AZD4320 | ChemieTek | Cat. #: CT-A4320 | in DMSO |
| Chemical compound, drug | Thapsigargin | Sigma-Aldrich | Cat. #:T9033 | in DMSO |
| Chemical compound, drug | Etoposide | Sigma-Aldrich | Cat. #: 33419-42-0 | in DMSO |
| Chemical compound, drug | Tunicamycin | Sigma-Aldrich | Cat. #: T7765 | in DMSO |
| Commercial assay or kit | TransIT-X2 | Mirus | Cat. #: Mir 6003 | Transfection reagent |
| Other | Cell Carrier-384, Ultra | PerkinElmer | Cat. #: 6057300 | for live cell imaging |
| Other | Non-binding surface, 96-well plate, black with clear bottom | Corning | Cat. #: 3881 | For recombinant protein and liposome assays critical to use non-binding plate |
| Other | Opera Phenix | PerkinElmer | Cat. #: HH14000000 | Automated spinning disc confocal microscope |
| Other | INO-FHS microscope | PMID:35442739 | Custom built by INO for Dr. David Andrews' lab | |
| Other | The Infinite M1000 | Tecan | Platereader for in vitro assays with recombinant proteins and liposome assays | |
| Gene (H. sapiens) | Bax | PMID:14522999, | GI: L22473.1 | For recombinant protein |
| Gene (H. sapiens) | Bcl-XL | PMID:18547146 | GI: Z23115.1 | For recombinant protein |
| Gene (H. sapiens) | PUMA | PMID:35442739 | GI: 27113 Addgene plasmid# 166739 |
|
| Gene (M. musculus) | BimL | PMID:30860026 | GI: AAD26594.1 | For recombinant BimL purification |
| Gene (M. musculus) | tBid | PMID:22464442 | GI: NM_007544.4 | for expression of VtBid in cells |
| Gene (M. musculus) | Bid | PMID:19062087 | GI: NM_007544.4 | Jean-Claude Martinou, SeronoPharmaceutical Institutte For purification of Bid |
| Software, algorithm | GraphPad Prism, version 6 | San Diego, California | RRID: SCR_002798 | Scientific graphing program, used to perform statistical analysis |
| Software, algorithm | MATLAB with toolboxes: Signal Processing, Curve Fitting, Image Processing, Version R2020a |
https://doi.org/10.5683/SP3/ZKXQW8 | RRID:SCR_00162 | https://www.mathworks.com/products/matlab.html |
| Software, algorithm | INO software package including INO-FHS Acquisition, INO_FHS_Analysis, and INO_FHS_Batch Analysis |
INO Client Release_r10357 package | ||
| Transfected Construct | mVenus-pEGFP-C1 | other | GI: KU341334.1 | Dr. Ray Truant (McMaster University). Backbone EGFP-C1 (Clonetech) |