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. 2023 May 1;25(5):672–684. doi: 10.1038/s41556-023-01136-6

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© The Author(s) 2023, corrected publication 2023

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Extended Data Fig. 3 — a, BioSorter sorting example on a synchronized population of adult dhs-3p::dhs-3::GFP worms according to their GFP fluorescence intensity (as a proxy for lipid droplet number). Optical density (extinction) and axial length (time of flight) of all particles measured by the sorter (left). Gate R3 was used to exclude bacterial debris and eggs and enrich for adult worms (15% of all particles). Individual worms from Gate R3 were sorted by GFP fluorescence intensity to separate the highest 10% fluorescent worms (Gate R4; right) and the lowest 10% fluorescent worms (Gate R1; right). b, Intestinal lipid droplet number, assessed in a synchronized population of middle-aged (adult day 6) dhs-3p::dhs-3::GFP worms, after manual sorting. Zoomed-in images of the intestine. Scale bar, 5 µm. Lipid droplet number is quantified in Fig. 4e.