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. 2023 May 1;25(5):672–684. doi: 10.1038/s41556-023-01136-6

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© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, Experimental set-up for sorting worms according to the fluorescence intensity of the DHS-3::GFP lipid droplet reporter in dhs-3p::dhs-3::GFP worms using a large-particle BioSorter. Created with BioRender.com. b,c, Number of intestinal lipid droplets, assessed by fluorescence, in a synchronized population of young adult (adult day 1) dhs-3p::dhs-3::GFP worms after sorting using a BioSorter. b, Zoomed-in images of the intestine. Scale bar, 5 µm. c, Number of lipid droplets in n = 15 worms for each condition. d, Worms sorted at young-adult age (adult day 1) with high numbers of lipid droplets live longer than worms with low numbers of lipid droplets; n ≥ 117 worms for each condition. e, Number of intestinal lipid droplets, measured by fluorescence, in a synchronized population of middle-aged (adult day 6) dhs-3p::dhs-3::GFP worms after manual sorting; n = 30 worms for each condition. Zoomed-in images are provided in Extended Data Fig. 3b. c,e, Each dot represents the number of lipid droplets in a 26 × 26 µm² area of the intestine of an individual worm. Data are the mean ± s.d. P values were determined using a two-tailed Mann–Whitney test. f, Worms sorted at middle age (adult day 6) with high numbers of lipid droplets live longer than worms with low numbers of lipid droplets; n ≥ 195 for each condition. d,f, Percentages of the median lifespan extension and P values, determined using a log-rank Mantel–Cox test, are indicated. c–f, Data are representative of two (c,d) or three (e,f) independent experiments. Source data are provided.

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