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. 2022 Aug 31;8(4):193–204. doi: 10.52601/bpr.2022.220005

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Fluorescence-annotated tomographic visualisation of an ER–mitochondrial membrane contact site. A When a diffraction-limited wide-field fluorescence micrograph is aligned to and superimposed on a tomogram slice, the blurred fluorescence pattern hardly presents any information. B When a superresolution fluorescence micrograph obtained with SMLM is aligned to and superimposed on the tomogram slice, the fluorescence marks an ER–mitochondrial membrane contact site. C In the position where fluorescence exists, the electron density (pink arrowheads) can be observed spanning the cleft of the membranes of the two organelles. D 3D segmentation of the ER membrane (cyan), the mitochondrial membranes (orange) and the density (yellow) between the ER membrane and the outer mitochondrial membrane. The insets in Panels C and D correspond to the same region. Watch Video S1 for an animated 3D presentation of Panels C and D. Scale bars: 100 nm