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. 2002 Apr 1;30(7):e31. doi: 10.1093/nar/30.7.e31

Figure 3.

Figure 3

Figure 3

Pyrogram demonstrating raw data obtained from multiplex Pyrosequencing using multiple templates obtained from multiplex PCR. Gel electrophoresis separation resolves two templates obtained from multiplex PCR (A). The left-hand lane represents the DNA marker, which is a 100 bp DNA ladder. Raw pyrogram data from each DNA template using a single primer are demonstrated in (B) and using multiple primers are shown in (C). Theoretical pattern expected from each nucleotide addition is demonstrated in the top panel of (C). The order of nucleotide dispensation is demonstrated below the pyrograms.