Fig. 2. Purkinje cell firing activity is abnormal in P10 Atoh1^Cre/+;Vglut2^fl/fl conditional knockout mice.

a Schematic of an in vivo single-unit extracellular Purkinje cell recording in an anaesthetized mouse. b Representative trace of a Purkinje cell recording in a control mouse (black and blue lines represent complex spikes). c Representative trace of a Purkinje cell recording in an Atoh1^Cre/+;Vglut2^fl/fl mouse (reddish purple, blue lines represent complex spikes). In b, c Inferior olive (IO) evoked complex spike (CS) in blue. The y-axis is constant across the panel. The x-axis (timescales) are the same for (b, c). d The frequency of Simple Spikes (SS) is different between control and Atoh1^Cre/+;Vglut2^fl/fl mice (p = 0.005; d = 0.81). e No differences were found in SS CV (spike pattern, p = 0.977; d = 0.04), f SS CV2 (spike regularity, p = 0.407; d = 0.26), g CS Frequency (p = 0.548, d = 0.22), or h CS CV (spike pattern, p = 0.171, d = 0.40). i Complex spikes occurred more regularly in control than in Atoh1^Cre/+;Vglut2^fl/fl mice CS CV2 (spike regularity, p = 0.039, d = 0.60). For d–i, large open circles represent the mouse average; small, closed circles represent the cell average; data points from control mice in black, data points from Atoh1^Cre/+;Vglut2^fl/fl mice in reddish purple. A linear mixed model analysis with genotype as a fixed variable and mouse number as a random variable was used to test for statistical significance in (d–i). Control: N_Mice = 5, n_Cells = 24 cells; Atoh1^Cre/+;Vglut2^fl/fl: N_mice = 5, n_Cells = 21. Source data and detailed statistical results are available and provided as a Source Data file. Panel (a) was adapted from White & Sillitoe, 2017, “Genetic silencing of olivocerebellar synapses causes dystonia-like behavior in mice,” Nature Communications²¹ under CC BY 4.0.