Fig. 5. Vglut1 expression in control and Atoh1^Cre/+;Vglut2^fl/fl mice.

a Schematic showing how conditional deletion of VgluT2 does not affect fast neurotransmission in VgluT1-expressing neurons. b Schematic of cerebellar connectivity and vesicular transporter expression for the glutamate subtypes (VgluT1 and VgluT2) in the cerebellar circuit in P7 control mice and Atoh1^Cre/+;Vglut2^fl/fl conditional knockout mice. c Same as b. but in adult mice. For a–c VGluT1 (blue); VGluT2 (orange); Control mice (black); Atoh1^Cre/+;Vglut2^fl/fl mice (reddish purple). d Expression of Vglut1 (green) with DAPI (purple, left) or tdTomato (purple, right) in the cerebellar nuclei (CN) and granule cell layer (gcl) of adult Atoh1^Cre/+;Rosa26^lsl-tdTomato mice. e Expression of Vglut1 (green) and DAPI (purple) in the CN and gcl of adult control mice and Atoh1^Cre/+;Vglut2^fl/fl mice. d, e Insets are 125 by 125 µm high magnification images. f VgluT1⁺ synapses (green) and DAPI (purple) in the molecular layer (ml) and gcl of P7 control and Atoh1^Cre/+;Vglut2^fl/fl mice, e-gcl = external granule cell layer containing actively proliferating granule cell precursor cells. g VgluT1⁺ synapses (green) and DAPI (purple) in the ml and gcl of adult control mice and Atoh1^Cre/+;Vglut2^fl/fl mice. d, e are shown at the same scale. f, g are shown at the same scale. Images are representative of N = 3 mice.