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. 2002 Apr 1;30(7):e32. doi: 10.1093/nar/30.7.e32

Figure 1.

Figure 1

Comparison of different glass supports and precipitation solutions for rat whole genome shotgun clones: lane 1, 500 ng pGEM plasmid template control; lanes 2 and 3, experiment A, ethanol precipitation of standard cleared alkaline lysates; lanes 4–11, 2-propanol precipitation: lanes 4 and 5, experiment B, using 212–150 µm acid-washed glass beads; lanes 6 and 7, experiment C, using <106 µm size-fractionated, acid-washed glass beads; lanes 8 and 9, experiment D, using a ground glass fiber filter (Whatman GF/C); lanes 10 and 11, experiment E, using a Millipore 1.2 µm glass fiber type C plate; lanes 12–13, experiment F, precipitation by 1.25 M NaCl, 10% PEG solution using 212–150 µm acid-washed glass beads. For each experiment 8.3% of the eluted product (5 µl) was loaded for the different glass support and precipitation solution variables prepared from 0.4 ml of bacterial cultures.