Fig. 2.

Inhibition of p53 enhances generation of hPSC-pancreatic progenitors. (A-B) Flow cytometry analysis and quantification showing the co-expression of PDX1 and NKX6.1 after treating the cells during stage 4 with DMSO, pifithrin-α, and pifithrin-μ. (C) Flow cytometry quantification of the co-expression of PDX1 and NKX6.1 after treating the cells during stage 4 with DMSO, and pifithrin-μ (10 μM and 20 μM). Flow cytometry analysis (D) and quantification (E) of PDX1 and NKX6.1 expression in DMSO or pifithrin-μ - treated pancreatic progenitors (n = 3). Flow cytometry analysis (F) and quantification (G) of CHGA and NKX6.1 expression in DMSO or Pifithrin-μ - treated cells (n = 3). Data are represented as mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001