Table 2.
Oligonucleotides used in this work.
| Primer name/pair | Direction | Sequence (5′–3′) |
|---|---|---|
| NEK1 exon 3 pair A (for NA and NB sgRNAs) | Fwd | ATGCAGTATTTGGCTTTCCAAAGC |
| Rev | GTCCAAACTAGATTAGGTTACAGAACAGC | |
| NEK1 exon 3 pair B (for NA and NB sgRNAs) | Fwd | TCATGTAAGTTTGCCTTTCTCTCC |
| Rev | GATGCTTCATGTTTGCCAATACTGC | |
| NEK1 exon 7 pair A (for NC sgRNAs) | Fwd | TCTGTGTGGATTGGCCTATTCTGG |
| Rev | ACAGATTTCAGGTGACAAGTAGTATGGG | |
| NEK1 exon 7 pair B (for NC sgRNAs) | Fwd | CATTCCTCTCCACCTAGACTCTGG |
| Rev | CCTATGCAAGTTCGAGCCAGC | |
| C21ORF2 exon 4 pair A (for CA and CB sgRNAs) | Fwd | TTTTCCTCACCTGTGAAGGAGGG |
| Rev | GGGATCAAGAGGAGACACAGAAGC | |
| C21ORF2 exon 4 pair B (for CA and CB sgRNAs) | Fwd | GCAACATGATATGTCCCCTGAGAGG |
| Rev | GAGACTTCACAGGACACATCTATGTCC |