Table 1.
Characteristics of antibodies used for Western blotting and microscopy.
| Antibody name | Dilution ratio | Species specificity | Source | Supplier | Cat. No |
|---|---|---|---|---|---|
| Phospho-DRP1 (Ser637) | 1:1,000a/1:200b | Mouse | Rabbit mAB | Cell Signaling | 4867S |
| Phospho-DRP1 (Ser616) | 1:1,000a/1:200b | Human | Rabbit mAB | Cell Signaling | 4494S |
| DRP1 | 1:1,000a/1:200b/1:200c | Mouse | Rabbit mAB | Cell Signaling | 8570S |
| Phospho-MFF (Ser146) | 1:1,000a/1:200b/1:200c | Mouse | Rabbit mAB | Cell Signaling | 49281 |
| MFF | 1:1,000a/1:200b | Mouse | Rabbit pAB | Cell Signaling | 86668S |
| HSL | 1:1,000 | Mouse | Rabbit pAB | Cell Signaling | 4107 |
| STAR | 1:10,000 | Mouse | Rabbit pAB | Abcam | ab96637 |
| CYP11A1 | 1:1,000 | Mouse | Rabbit mAB | Cell Signaling | 14217 |
| HSD3B | 1:1,000 | Mouse | Rabbit mAB | A gift from Dr. Ian Mason | |
| TOM20 | 1:200c | Mouse | Rabbit mAB | Cell Signaling | 42406S |
| VDAC1/Porin | 1:1,000 | Mouse | Rabbit pAB | Abcam | Ab15895 |
| IkBα | 1:1,000 | Mouse | Rabbit pAB | Santa Cruz Biotechnology, Inc. | Sc-847 |
| Cyt C | 1:1,000 | Bovine | Mouse mAB | Abcam | ab110325 |
| Phospho-AMPKα (Thr172) | 1:1,000 | Mouse | Rabbit pAB | Cell Signaling | 2535S |
| AMPKα | 1:1,000 | Mouse | Rabbit mAB | Cell Signaling | 2532S |
| Phospho-PINK1 (Ser228) | 1:1,000 | Mouse | Rabbit pAB | Thermo Fisher Scientific | PA5-105356 |
| PINK1 | 1:1,000 | Human | Rabbit mAB | Cell Signaling | 6946S |
| Phospho-PARKIN (Ser65) | 1:1,000 | Mouse | Rabbit pAB | Thermo Fisher Scientific | PA5-114616 |
| PARKIN | 1:2,000 | Mouse | Mouse mAB | Abcam | ab77924 |
| Phospho-ULK1 (Ser555) | 1:000 | Mouse | Rabbit mAB | Cell Signaling | 5869S |
| ULK1 | 1:000 | Mouse | Rabbit mAB | Cell Signaling | 8054S |
| LC3B | 1:000 | Mouse | Rabbit mAB | Cell Signaling | 3868S |
| LysoPrime Green | 1 μM | Dojindo | L261 | ||
| Mtphagy Dye | 100 nM | Dojindo | MT02-10 | ||
| BODIPY 493/503 | 20 µM | Thermo Fisher Scientific | D3922 | ||
| MitoTracker Red FM | 200nM | Thermo Fisher Scientific | M22425 | ||
| ACTB | 1:5,000 | Bovine | Mouse mAB | Sigma Life Science | A5441 |
| Beta-tubulin | 1:5,000 | Bovine | Mouse mAB | Sigma Life Science | T4026 |
| Alpha-tubulin | 1:200 | Bovine | Mouse mAB | Abcam | ab7291 |
| HRP-linked | 1:10,000 | Anti-rabbit | Jackson ImmunoResearch | 111035003 | |
| HRP-linked | 1:10,000 | Anti-mouse | Jackson Laboratory | 115035205 | |
| DyLight 405 | 1:500 | Anti-mouse | Jackson Laboratory | 115-475-166 | |
| Alexa Fluor 488 | 1:500 | Anti-mouse | Invitrogen | A32723 | |
| Alexa Fluor 594 | 1:500 | Anti-rabbit | Invitrogen | A-11032 | |
| Alexa Fluor 647 | 1:500 | Anti-biotin | Biolegend | 405237 |
Dilution used for Western blotting.
Dilution used for confocal microscopy.
Biotinylated antibody.
Dynamin-related protein 1 (DRP1); mitochondrial fission factor (MFF); hormone sensitive lipase (HSL); steroidogenic acute regulatory protein (STAR); cholesterol side-chain cleavage enzyme (CYP11A1); 3beta-Hydroxysteroid dehydrogenase (HSD3B); mitochondrial import receptor subunit 20 (TOM20); voltage-dependent anion-selective channel 1 (VDAC1); NF kappa B inhibitor alpha (IkBalpha); cytochrome complex (Cyt C); AMP-activated protein kinase (AMPK); PTEN-induced kinase 1 (PINK1); Parkin RBR E3 ubiquitin-protein ligase (PAKIN); Unc-51-like autophagy activating kinase (ULK1); autophagy marker Light Chain 3 (LC3); beta-actin (ACTB; loading control); beta-tubulin (TUBB; loading control); alpha-tubulin (TUBA).