Figure 4.

Global PICK1-deficient mice display impaired insulin secretion during vehicle conditions, but not after HFD or GLT exposure. (A, B) During the OGTT, insulin was measured −60 (basal) minutes and 15 minutes for mice on chow or HFD (n ≥ 8). (C) Representative images of pancreatic tissue from WT and PICK1-deficient mice following HFD, immunostained for insulin (magenta) or glucagon (cyan). Scale bar = 100 μm. (D) Quantification of the insulin immunosignal (E) islet area and (F) number of islets (n ≥ 5). (G, H) Glucose-stimulated insulin secretion was conducted on islets isolated from PICK1-deficient and WT mice on chow diet and treated with vehicle or GLT conditions, respectively. Data shown as dot plot with mean ± SEM (n = 3). Statistical significance was determined using 2-way ANOVA with Sidak's multiple comparisons test or unpaired t test. *P < .05, ***P < .001.