Figure 5.

βPICK1-KO mice in vivo and ex vivo display decreased insulin secretion during normal conditions. βPICK1-KO and WT mice were kept on chow diet and (A) BW measured following 21 weeks (n ≥ 11). (B) OGTT was carried out in fasted mice and (C) during the OGTT insulin was measured −60 (basal) minutes and at 15 minutes. (D) The difference from (C) in plasma insulin from −60 minutes to 15 minutes. (E, F) Glucose-stimulated insulin secretion was conducted on islets isolated from βPICK1 KO and WT mice and treated with vehicle or GLT, respectively. Data are shown as dot plot with mean ± SEM (n ≥ 6). Statistical significance was determined using 2-way ANOVA with Sidak's multiple comparisons test or unpaired t test. *P < .05, ***P < .001.