Table 1.
Reportable range and geometric mean of antifungal agents used in this study and analysis on the agreement of D48 and SYO detection results.
| Antifungal Agents | Reportable Range (μg/mL) | Geometric Mean (μg/mL) |
% | Cohen’s kappa coefficient [95% confidence interval] |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| D48 | SYO | D48 | SYO | EA [NEA/NT] |
CA [NCA/NCBs] |
VMD [NVMD/NR] |
MD [NMD/NS] |
mD [NmD/NT] |
||
| Amphotericin B | 0.03–16 | 0.12-8 | 0.76 | 0.54 | 95.07 [135/142] | — | — | — | — | 0.47 [0.11–0.83] |
| Itraconazole | 0.016–8 | 0.015-16 | 0.39 | 0.18 | 97.89 [139/142] | — | — | — | — | 0.85 [0.57–1.14] |
| 5-Flucytosine | 0.06–64 | 0.06-64 | 0.09 | 0.31 | 92.25 [131/142] | — | — | — | — | — |
| Caspofungin | 0.03–32 | 0.008-8 | 0.28 | 0.11 | 92.96 [132/142] | 92.59 [73/78] | 0 | 0 | 6.41 [5/78] | 0.83 [0.70–0.97] |
| Fluconazole | 0.25–256 | 0.12-256 | 1.84 | 2.41 | 98.59 [140/142] | 92.30 [48/52] | 0 | 0 | 7.69 [4/52] | 0.89 [0.82–0.97] |
| Voriconazole | 0.008–8 | 0.008-8 | 0.12 | 0.08 | 97.18 [138/142] | 87.93 [51/58] | 0 | 0 | 12.07 [7/58] | 0.81 [0.70–0.92] |
| Anidulafungin | 0.016–16 | 0.015-8 | 0.36 | 0.09 | 90.85 [129/142] | 93.59 [73/78] | 0 | 2.60 [2/77] | 3.85 [3/78] | 0.83 [0.69–0.97] |
| Micafungin | 0.03-32 | 0.008-8 | 0.06 | 0.07 | 98.59 [140/142] | 98.72 [77/78] | 0 | 0 | 1.28 [1/78] | 0.93 [0.85–1.00] |
| Posaconazole | 0.016–2 | 0.008-8 | 0.15 | 0.12 | 86.62 [123/142] | — | — | — | — | 0.06 [-0.16–0.27] |
EA, essential agreement; minimal inhibitory concentration (MIC) result obtained with Droplet 48 (D48) that is within two two-fold dilution of the MIC value determined by the Sensititre YeastOne (SYO) method. NEA is the number of tests that resulted in EA; CA, categorical agreement; agreement of susceptible, intermediate, susceptible-dose-dependent, and resistant results between the D48 and SYO methods. NCA is the number of tests that result in CA, NCBs is the number of tests that have the Clinical and Laboratory Standards Institute clinical breakpoints, very major discrepancy (VMD) is the major discrepancy, and VMD = (NVMD × 100)/NR. NVMD is the number of tests that result in a VMD, and NR is the number of resistant microbial isolates as determined by SYO; MD, major discrepancy; MD = (NMD • 100)/NS. NMD is the number of tests that resulted in an MD; NS is the number of susceptible microbial isolates as determined by SYO; mD, minor discrepancy, mD = (NmD • 100)/NT, where NmD is the number of tests that result in an mD, and NT is the total number of isolates tested. If the MIC value of a method was identified as a range value (e.g., ≤2 or >4), the MIC value of another method that differed from its critical value within ±2 two-fold dilutions or within its range was also considered consistent (e.g., 8 vs. >2, 0.016 vs. ≤2, 2 vs. ≤4; 32 vs. >4); a horizontal thin line indicates no data available.