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Quantitative murein hydrolase assays of the lrgAB mutant. Aliquots (100 μg) of the extracellular proteins used in Fig. 3 were added to a 1-mg/ml suspension of S. aureus cells, and the turbidity was monitored for 6 h. Strains used in this analysis were RN6390(pRB374) (○), RN6390(pRB-lrgAB) (▾), KB345(pRB374) (▿), and KB345(pRB-lrgAB) (■). A negative control (●) in which no murein hydrolase was added to the cell suspension was also performed.
