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. 2000 Apr;182(7):1802–1811. doi: 10.1128/jb.182.7.1802-1811.2000

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Copyright © 2000, American Society for Microbiology

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FIG. 1 — Effects of different compounds on iniBAC promoter activity as measured by luciferase assays of integrated transcriptional fusion plasmids. Induction is shown after incubation with compounds for 24 h (grey bars) or 48 h (black bars). Final concentrations are indicated in micrograms per milliliter. Error bars represent 95% confidence intervals. (A) INH-susceptible BCG^S(pG4697-6) containing the iniBAC promoter fused to lacZ and fflux genes [-Pro indicates assays performed with BCG^S(pG1697-3), which contains the same construct, except that the transcriptional fusion was performed by inserting the promoter in the opposite orientation from the coding region]. (B) INH-susceptible BCG(pMKB15) containing the L5 phage p_L promoter fused to the fflux gene. (C) INH-resistant BCG^R(pG4697-6) containing the iniBAC promoter fused to lacZ and fflux genes.

FIG. 1 — Effects of different compounds on iniBAC promoter activity as measured by luciferase assays of integrated transcriptional fusion plasmids. Induction is shown after incubation with compounds for 24 h (grey bars) or 48 h (black bars). Final concentrations are indicated in micrograms per milliliter. Error bars represent 95% confidence intervals. (A) INH-susceptible BCG^S(pG4697-6) containing the iniBAC promoter fused to lacZ and fflux genes [-Pro indicates assays performed with BCG^S(pG1697-3), which contains the same construct, except that the transcriptional fusion was performed by inserting the promoter in the opposite orientation from the coding region]. (B) INH-susceptible BCG(pMKB15) containing the L5 phage p_L promoter fused to the fflux gene. (C) INH-resistant BCG^R(pG4697-6) containing the iniBAC promoter fused to lacZ and fflux genes.