FIG. 5.

Testing of the iniBAC operon in different mycobacterial species. Mycobacterial species were tested by low-stringency Southern blot hybridization for the presence of the iniA and iniB genes: M. marinum (lane 1), H37ra (lane 2), M. tuberculosis strains Erdman (lane 3) and H37Rv (lane 4), M. avium (lane 5), BCG (lane 6), M. smegmatis (lane 7), M. nonchromogenicum (lane 8), and M. microti (lane 9). (A) Agarose gel showing PvuII digests of chromosomal DNA. (B) Southern blot of the gel in panel A hybridized with the iniA gene probe. (C) Hybridization with the M. tuberculosis 16S RNA gene probe. (D) Hybridization with the iniB gene.