TABLE 2.
Primers used in this study
| Name | Sequence, added restriction sites (underlined), spacer sequences (lowercase) | Positiona |
|---|---|---|
| iigBPT | GATCATCACGGCTACGACATCCAC | 409142–409165 |
| iigBPIIB | TGACCGCCGAAACCACCGCTTGAC | 409745–409722 |
| iigBproxhoT | GGAACTCGAGATCATCACGGCTACGACATCCAC | 409143–409165 |
| iigB2proxhoB | TCATCTCGAGTTCCCTTCAATCGAAGAAGC | 409353–409334 |
| iniBproEcoT | CCGTCGAATTCGATCATCACGGCTACGACATCCAC | 409142–409165 |
| iniBproSalB | TCATGTCGACTTCCCTTCAATCGAAGAAGCTGTT | 409353–409330 |
| iniBproEcoT20 | CGGCTGAATTCTCCACGGATAAGTTCCGGACCGGC | 409161–409184 |
| iniBproEcoT43 | TTCCGGAATTCCGTAGGGGTGCCCCATTTCCCCTA | 409184–409207 |
| iniBproEcoT65 | CCCATGAATTCTAATCCCCTAACGCGGCGGCCAGG | 409206–409229 |
| iniBproEcoT90 | GGCGGGAATTCCGATCCCGATAGGTGTTTGGCCGG | 409231–409254 |
| iniBproEcoT113 | TTGTTGAATTCGCTTGCGGATCAGACCCCGATTTC | 409254–409277 |
| iniBproEcoT134 | CAGACGAATTCTTCGGGGTGAGGCGGAATCCATAG | 409275–409298 |
| iniBproEcoT154 | AGGCGGAATTCATAGCGTCGATGGCACAGCGCCGG | 409295–409318 |
| iniBproEcoT175 | ATGGCGAATTCCCGGTCACGCCGGCGAACAGCTTC | 409315–409338 |
| iniBproEcoT189 | TCACGGAATTCAACAGCTTCTTCGATTGAAGGGAA | 409330–409348 |
| iniBproSalB193 | AATCGGTCGACCTGTTCGCCGGCGTGACCGGCGCT | 409334–409311 |
| iniBproSalB173 | GGCTGGTCGACCGCTGTGCCATCGACGCTATGGAT | 409314–409291 |
| iniBproSalB193-spa | TTAAGGTCGACggtctccagtcgctcgactCTGTTCGCCGGCGTGACCGGCGCT | 409334–409311b |
| iniBproSalB148 | TCGGTGTCGACCGCCTCACCCCGAAATCGGGGTCT | 409288–409265 |
| iniBprimer ext-1 | CTGCGGAACAGGCTCAGGATGTAA | 409400–409377 |
| iniBprimer ext-2 | GCCCGTCCCGGAGCGGCAACGAAC | 409442–409419 |
| iniBprimer ext-3 | CGATAAGCGAGGTCATCTTCAT | 409375–409354 |
| iniART-T | GCGCTGGCGGGAGATCGTCAATG | 411552–411574 |
| iniART-B | TGCGCAGTCGGGTCACAGGAGTCG | 412043–412020 |
| B1A | ACATACGTACAACTCGAGAGAGGCACTTCGAGATGGCCTT | NA |
| B4B | ACAATGCATTCAGGGGGCGGGTAGAGTGCGCGGTTTCCA | NA |
| HYG1 | TGCCAACTAGTGCCCGTACCCTGTGAATAGA | NA |
| HYG2 | GAGCAAAGCTTGCGTACGATCGACTGCCAGG | NA |
a
Position for nucleotides after the added restriction site (if any). NA, not applicable.
b
Position for nucleotides after the spacer sequence.