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SDS-PAGE analysis of the reaction of apo-NCS with calf thymus histones. Reaction conditions: 50 mM Tris-HCl buffer (pH 7.5), histones (1 mg/ml), and recombinant (500 μg/ml) (A) or natural (100 μg/ml) apo-NCS (B) in a total volume of 100 μl. Numbers are incubation times (hours) for each sample.
